FOXA1 antibody

Catalog No. ABIN6296010

This anti-FOXA1 antibody is a Mouse Monoclonal antibody detecting FOXA1 in WB, IHC, FACS and IF. Suitable for Human and Rat.

Quick Overview for FOXA1 antibody (ABIN6296010)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-FOXA1 Antibody

Purpose: Mouse anti-Human/Rat FOXA1 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this FOXA1 antibody.

Immunogen: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this FOXA1 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: Hepatocyte nuclear factor 3-alpha

Background:

Target Description: The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Gene Symbol: FOXA1///HNF3A///TCF3A

Gene ID: 3169

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis